Human Interleukin 4 (IL4) ELISA Kit

SKU: orb775017

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4(IL4).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.6 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BSF1 ELISA Kit, BCGF1 ELISA Kit, B Cell Stimulatory Factor 1 ELISA Kit, Lymphocyte stimulatory factor 1 ELISA Kit, B Cell Growth Factor ELISA Kit, Binetrakin ELISA Kit, Pitrakinra ELISA Kit

Quick Database Links

UniProt

P05112

References

R Paduch, M Klatka, P Pieniądz, I Wertel, A Pawłowska, J Klatka Reciprocal Interactions of Human Monocytes and Cancer Cells in Co-Cultures In Vitro Current Issues in Molecular Biology, (2024)

PubMed:

Applications

ELISA

Reactivity

Human