Human Interleukin 37 (IL37) ELISA Kit

SKU: orb775208

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Zeta(IL1z).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-37 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 3.3 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IL1 Zeta,IL1F7 ELISA Kit, IL37 ELISA Kit, FIL1 ELISA Kit, FIL1(ZETA) ELISA Kit, FIL1Z ELISA Kit, IL-1F7 ELISA Kit, IL-1H4 ELISA Kit, IL-1RP1 ELISA Kit, IL1H4 ELISA Kit, IL1RP1 ELISA Kit, Interleukin 37 ELISA Kit, Interleukin-1-Related Protein ELISA Kit, Interleukin 1 Family, Member 7 ELISA Kit

Quick Database Links

UniProt

Q9NZH6