Human Interferon Induced Helicase C Domain Containing Protein 1 (IFIH1) ELISA Kit

SKU: orb781095

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Induced Helicase C Domain Containing Protein 1(IFIH1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFIH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFIH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFIH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFIH1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.12 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Hlcd ELISA Kit, IDDM19 ELISA Kit, MDA5 ELISA Kit, Helicard ELISA Kit, Clinically amyopathic dermatomyositis autoantigen 140 kDa ELISA Kit, Melanoma differentiation-associated protein 5 ELISA Kit, Murabutide down-regulated protein ELISA Kit

Quick Database Links

UniProt

Q9BYX4