Human Interferon Alpha/Beta Receptor 2 (IFNa/bR2) ELISA Kit

• Catalog Number: orb777413
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha/Beta Receptor 2(IFNa/bR2).
• Reactivity: Human
• Concentration: 40 ng/mL
• UniProt ID: P48551
• Tested applications: ELISA
• Application notes: standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNa/bR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNa/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNa/bR2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.25 ng/mL
• Sample Types: Serum, plasma, urine and other biological fluids.
• Alternative names: IFNαR2 ELISA Kit, IFN-alpha-REC ELISA Kit, IFN-R E
• Research Area: Infectious Disease & Virology
• Note: For research use only
• Expiration Date: Please enquire.