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| Catalog Number | orb782672 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Parathyroid Hormone(PTH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Parathyroid Hormone(PTH). |
| Reactivity | Human |
| Concentration | 200 pg/mL |
| Tested applications | ELISA |
| Application notes | standard: 200 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 3.13-200 pg/mL |
| Sensitivity | 0.9 pg/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | iPTH ELISA Kit, Intact Parathyroid Hormone ELISA K Read more... |
| Note | For research use only |
