Human Inhibitor of Kappa-Light Polypeptide Gene Enhancer in B-Cells Kinase Gamma (IkBKg) ELISA Kit

SKU: orb779435

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma(IkBKg).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IkBKg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IkBKg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IkBKg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IkBKg in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.066 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IP ELISA Kit, FIP-3 ELISA Kit, FIP3 ELISA Kit, Fip3p ELISA Kit, IKK-gamma ELISA Kit, IP1 ELISA Kit, IP2 ELISA Kit, NEMO ELISA Kit, NF-Kappa-B Essential Modulator ELISA Kit, Incontinentia Pigmenti ELISA Kit, IkB kinase-associated protein 1 ELISA Kit, NF-kappa-B essential modifier ELISA Kit