You have no items in your shopping cart.
Cart summary
| Catalog Number | orb1736572 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Infliximab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Infliximab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Infliximab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Infliximab in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 4.69-300 ng/mL |
| Assay Time | 3.5h |
| Concentration | 300 ng/mL |
| Sensitivity | 1.77 ng/mL |
| Alternative names | infliximab Read more... |
| Note | For research use only |
| Application notes | standard: 300 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Infliximab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Infliximab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Infliximab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Infliximab in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
| Expiration Date | Please enquire. |
