Cart summary

You have no items in your shopping cart.

Human Inducible T-Cell Co Stimulator Ligand (ICOSLG) ELISA Kit

Catalog Number: orb778334

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb778334
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inducible T-Cell Co Stimulator Ligand(ICOSLG).
ReactivityHuman
Concentration10 ng/mL
UniProt IDO75144
Tested applicationsELISA
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ICOSLG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ICOSLG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ICOSLG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ICOSLG in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.065 ng/mL
Sample Typestissue homogenates, cell lysates and other biological fluids
Alternative namesCD275 ELISA Kit, B7-H2 ELISA Kit, B7H2 ELISA Kit,
Read more...
NoteFor research use only
Human Inducible T-Cell Co Stimulator Ligand (ICOSLG) ELISA Kit