Human Interleukin 6 (IL6) ELISA Kit

SKU: orb775021

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6(IL6).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 3.3 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MGI2-A ELISA Kit, MGI2A ELISA Kit, HGF ELISA Kit, BSF2 ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, B-Cell Stimulatory Factor-2 ELISA Kit, Hybridoma/Plasmacytoma Growth Factor ELISA Kit, Hepatocyte Stimulating Factor ELISA Kit, Cytotoxic T-Cell Differentiation Factor ELISA Kit

Quick Database Links

UniProt

P05231

References

Anita Gąsiorowska 1ORCID,Marek Romanowski 1,Ewa Walecka-Kapica 1,Aleksandra Kaczka 1,Cezary Chojnacki 1ORCID,Milena Padysz 1,Marta Siedlecka 1,Julia Banasik 1,Aleksandra Sobolewska-Włodarczyk 1,Maria Wiśniewska-Jarosińska 1,Joanna B. Bierła 2ORCID,Nize Otaru 3,Bożena Cukrowska 4,*ORCID andRobert E. Steinert Robert E. Steinert SciProfilesScilitPreprints.orgGoogle Scholar Efficacy and Safety of a Mixture of Microencapsulated Sodium Butyrate, Probiotics, and Short Chain Fructooligosaccharides in Patients with Irritable Bowel Syndrome—A Randomized, Double-Blind, Placebo-Controlled Study Journal of Clinical Medicine, (2024)

PubMed:

Applications

ELISA

Reactivity

Human

Jasmin Bayer,Nicole Karoline Petersen,Jeanine Veruschka Hess,Yvonne Jockel-Schneider,Petra Högger * Impact of a Dietary Supplementation with French Maritime Pine Bark Extract Pycnogenol® on Salivary and Serum Inflammatory Biomarkers During Non-Surgical Periodontal Therapy—A Randomized Placebo-Controlled Double-Blind Trial preprints.org, (2025)

PubMed:

Applications

ELISA

Reactivity

Human