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Human IL4 Protein, His Tag
Description
Images & Validation
−Key Properties
−| Source | E.coli |
|---|---|
| Expression System | HEK293 |
| Target | IL4 |
| Reactivity | Human |
| Tag | C-6×His Tag |
| Molecular Weight | The protein has a predicted molecular mass of 15.8 kDa after removal of the signal peptide. The apparent molecular mass of IL4-His is approximately 15-25 kDa due to glycosylation. |
| Expression Region | IL4(His25-Ser153) 6×His tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.0. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Disclaimer | For research use only |
Alternative Names
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Greater than 95% as determined by reducing SDS-PAGE.
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UniProt Details
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Protocol Information
Human IL4 Protein, His Tag (orb1743263)
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