Human Interleukin 33 (IL33) ELISA Kit

SKU: orb776592

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 33(IL33).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL33. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL33. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL33, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL33 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.1 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

DV27 ELISA Kit, C9ORF26 ELISA Kit, IL1F11 ELISA Kit, NFHEV ELISA Kit, Interleukin-1 Family, Member 11 ELISA Kit, Nuclear factor from high endothelial venules ELISA Kit

Quick Database Links

UniProt

O95760

References

P Cieplewicz-Guźla, MJ Kowalczyk The role of selected cytokines from the interleukin 1 family in the pathophysiology of morphea. researchsquare.com, (2025)

PubMed:

Applications

ELISA

Reactivity

Human