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Catalog Number | orb391952 |
---|---|
Category | Proteins |
Description | Recombinant human IL1RA protein is produced by our E. coli expression system and the target gene encoding Arg26-Glu177 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE. |
Target | IL1RN |
Entrez | 3557 |
UniProt ID | P18510 |
Protein Sequence | MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 7.5. |
Alternative names | IL1F3; IL1RA; Interleukin-1 receptor antagonist pr Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, FC, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
> 96% as determined by SDS-PAGE and HPLC. | |
17.1 kDa | |
E.Coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 62.3 kDa after removal of the signal peptide.The apparent molecular mass of IL1RA-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Greater than 95% as determined by SDS-PAGE. | |
17.26 kDa | |
E.coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 37.5 kDa after removal of the signal peptide. The apparent molecular mass of IL1RA-His is approximately 35-70 kDa due to glycosylation. | |
Mammalian |