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Catalog Number | orb752946 |
---|---|
Category | Proteins |
Description | IL1A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (113-271 a.a) and having a molecular mass of 22.4kDa. IL1A is fused to a 38 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered colorless solution. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Protein Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSA PFSFLSNVKY NFMRIIKYEF ILNDALNQSI IRANDQYLTA AALHNLDEAV KFDMGAYKSS KDDAKITVIL RISKTQLYVT AQDEDQPVLL KEMPEIPKTI TGSETNLLFF WETHGTKNYF TSVAHPNLFI ATKQDYWVCL AGGPPSITDF QILENQA |
Source | E.coli |
Biological Activity | Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is less or equal to 0.04 ng/ml. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | IL1A protein solution (1mg/ml) contains 20mM Tris-HCl (pH 7.5) and 10% glycerol. |
Alternative names | Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
18.0 kDa | |
E.Coli |
Greater than 90% as determined by SDS-PAGE. | |
45 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
22 kDa | |
E.coli |