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Catalog Number | orb391933 |
---|---|
Category | Proteins |
Description | Recombinant human IL17F protein is produced by our E. coli. It is a disulfide-linked homodimer of 30.1 kDa consisting of two non-glycosylated polypeptide chains with 134 amino acids each. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, pH 7.4. |
Purity | Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE. |
Target | IL17F |
Entrez | 112744 |
UniProt ID | Q96PD4 |
Protein Sequence | MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, pH 7.4. |
Alternative names | Interleukin-17F; IL-17F; Cytokine ML-1 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
16.8 kDa | |
Human IL-17F (H161R), His Tag (orb334906) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1 (H161R)). |
> 95% as determined by SDS-PAGE and HPLC. | |
15 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
14.9 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
15.96 kDa | |
Mammalian cell |
Biotin | |
95% | |
77.6 kDa | |
Biotinylated Human IL-17 RC, Fc, (orb1291791) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - His 465 (Accession # Q8NAC3-2). |
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