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Catalog Number | orb612149 |
---|---|
Category | Proteins |
Description | Human IL-8 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Ser 28 - Ser 99 (Accession # P10145-1). |
Target | IL-8 |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_000575.1 |
UniProt ID | P10145 |
MW | 10.3 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 10.3 kDa. The protein migrates as 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 0.1 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-8 Protein, His Tag, premium grade (Cat. No. ) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL-8 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested). |
Expression Region | Ser 28 - Ser 99 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_000575.1 |
Immobilized Human IL-8 Protein, His Tag, premium grade (Cat. No. orb612149) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL-8 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
SDS-PAGE, WB | |
Unconjugated | |
Purity greater than 95% as determined by analysis of RP-HPLC and reducing and non-reducing SDS-PAGE against a known standard. |
99.10% | |
9.8 kDa (predicted) |
98.00% | |
35.8 kDa (predicted) |
98.00% | |
19.6 kDa (predicted) |