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Human IL-7 R alpha Protein, mFc Tag

Catalog Number: orb402998

DispatchUsually dispatched within 5-10 working days
$ 640.00
Catalog Numberorb402998
CategoryProteins
DescriptionHuman IL-7 R alpha, Mouse IgG2a Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1 (I66T,V138I)).
ReactivityHuman
TagC-mFc(mIgG2a)
Form/AppearancePowder
Purity90%
ConjugationUnconjugated
MW52.0 kDa
TargetIL-7 R alpha
UniProt IDP16871
Protein SequenceGlu 21 - Gly 236
Expression SystemHEK293
Biological OriginHuman
Biological ActivityCaptured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. ) on CM5 chip via anti-mouse antibodies surface can bind Human IL-7, Tag Free (Cat. No. IL7-H4219) with an affinity constant of 13.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Expression RegionGlu 21 - Gly 236
Endotoxins1.0 EU per μg
Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Buffer/PreservativesTris with Glycine, Arginine and NaCl, pH 7.5
NoteFor research use only
Application notesThis protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 75-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date6 months from date of receipt.
Human IL-7 R alpha Protein, mFc Tag

Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. orb402998) on CM5 chip via anti-mouse antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. orb257603) with an affinity constant of 13.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).