Human IL-7

SKU: orb3002429

Description

Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encoding Asp26-His177 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: BLI: Loaded Human IL-7RA-His(Cat#orb2993835) on HIS1K Biosensor, can bind Human IL-7(Cat#orb3002429) with an affinity constant of 15.04 nM as determined in BLI assay. Loaded Human IL-7RA-Fc-His(Cat#orb2994563) on HIS1K Biosensor, can bind Human IL-7(Cat#orb3002429) with an affinity constant of 18.59 nM as determined in BLI assay. Loaded Mouse IL-7RA-Fc(Cat#orb2994406) on Protein A Biosensor, can bind Human IL-7(Cat#orb3002429) with an affinity constant of 29.2 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.02-0.08 ng/ml.
• Tag: Tag free
• Molecular Weight: Predicted: 17.5 KDa. Observed: 18 KDa, reducing conditions
• Expression Region: Asp26-His177
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
• Endotoxins: < 0.01 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Interleukin-7; IL-7; IL7

Quick Database Links

UniProt

P13232