Human IL-6 R alpha Protein, His Tag

SKU: orb257625

Description

Human IL-6 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1).

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.4 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: IL-6 R alpha
• Reactivity: Human
• Tag: C-6×His
• Molecular Weight: 39.4 kDa
• Expression Region: Leu 20 - Pro 365
• Purity: 95%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Disclaimer: For research use only

SDS-PAGE analysis of Human IL-6 R alpha protein

Line graph illustrates about the sensitivity range of Human IL-6 R alpha protein using different concentrations

Loaded Human IL-6 R alpha, His Tag (Cat. No. orb257625) on HIS1K Biosensor, can bind Human IL-6, premium grade (Cat. No. orb257601) with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Quick Database Links

Gene Symbol

IL-6 R alpha

UniProt

NP_000556.1

References

Tanemura, Yutaro et al. Easy and rapid binding assay for functional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system Biology (Basel), 4, 161-172 (2015)

PubMed: 25738808