Human IL-6 R alpha Protein, His Tag

• Catalog Number: orb257625
• Category: Proteins
• Description: Human IL-6 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1).
• Target: IL-6 R alpha
• Conjugation: Unconjugated
• Tag: C-6×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• Protein Sequence: NP_000556.1
• UniProt ID: P08887
• MW: 39.4 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.4 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Loaded Human IL-6 R alpha, His Tag (Cat. No. ) on HIS1K Biosensor, can bind Human IL-6, premium grade (Cat. No. IL6-H4218) with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: Leu 20 - Pro 365
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_000556.1

References

Tanemura, Yutaro et al. Easy and rapid binding assay for functional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system Biology (Basel), 4, 161-172 (2015)

PubMed: 25738808

SDS-PAGE analysis of Human IL-6 R alpha protein

Line graph illustrates about the sensitivity range of Human IL-6 R alpha protein using different concentrations

Loaded Human IL-6 R alpha, His Tag (Cat. No. orb257625) on HIS1K Biosensor, can bind Human IL-6, premium grade (Cat. No. orb257601) with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).