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Item 1 of 3
| Catalog Number | orb257625 |
|---|---|
| Category | Proteins |
| Description | Human IL-6 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1). |
| Reactivity | Human |
| Tag | C-6×His |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 39.4 kDa |
| Target | IL-6 R alpha, CD126 |
| UniProt ID | P08887 |
| Source | Human IL-6 R alpha, His Tag (orb257625) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-6 R alpha, His Tag on HIS1K Biosensor, can bind Human IL-6, premium grade with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Leu 20 - Pro 365 |
| Endotoxins | 1.0 EU per μg |
| NCBI | NP_000556.1 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | IL6R,CD126,IL-6R-1,IL-6RA,IL6RA,IL-6R-alpha,IL6RQ, Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.4 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Tanemura, Yutaro et al. Easy and rapid binding assay for functional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system Biology (Basel), 4, 161-172 (2015)
SDS-PAGE analysis of Human IL-6 R alpha protein
Line graph illustrates about the sensitivity range of Human IL-6 R alpha protein using different concentrations
Loaded Human IL-6 R alpha, His Tag (Cat. No. orb257625) on HIS1K Biosensor, can bind Human IL-6, premium grade (Cat. No. orb257601) with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Recombinant Human IL-6 R alpha Protein, His Tag [orb1550904]
> 92% by SDS-PAGE.
Recombinant Human IL-6 R alpha Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Leu20-Asp358) of human IL-6 R alpha (Accession #NP_000556.1) fused with a 6��His Tag at the C-terminus.
50 μg, 100 μg
