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| Catalog Number | orb612145 |
|---|---|
| Category | Proteins |
| Description | Human IL-33 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1). |
| Target | IL-33 |
| Conjugation | Unconjugated |
| Tag | N-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| UniProt ID | O95760 |
| MW | 20.2 kDa |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.1 EU per μg |
| Expression Region | His 109 - Thr 270 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

Loaded Human IL-1RL1, Fc Tag (Cat. No. orb257583) on Protein A Biosensor, can bind with Human IL-33 Protein, His Tag, premium grade (Cat. No. orb612145) an affinity constant of 1.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
>90% as determined by SDS-PAGE. | |
20.3 kDa |
>90% as determined by SDS-PAGE | |
19.3 kDa |
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