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Human IL-3 Protein, His Tag

Catalog Number: orb1087548

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb1087548
CategoryProteins
DescriptionHuman IL-3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1).
TargetIL-3
ConjugationUnconjugated
TagC-10×His
ReactivityHuman
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Purity90%
Protein SequenceNP_000579.2
UniProt IDP08700
MW17.0 kDa
Application notesThis protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression SystemHEK293
Endotoxins0.3 EU per μg
Biological OriginHuman
Biological ActivityLoaded Human IL-3 R alpha, Fc Tag (Cat. No. ILA-H5252) on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. ) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Expression RegionAla 20 - Phe 152
Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
NoteFor research use only
NCBINP_000579.2
Human IL-3 Protein, His Tag

Loaded Human IL-3 R alpha, Fc Tag on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. orb1087548) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Mouse IL-3 Protein, His Tag [orb1101195]

    Unconjugated

    90%

    17.6 kDa

    1 mg, 100 μg
  • Human CD123 Protein, hFc-His Tag [orb689364]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 80-90 kDa after removal of the signal peptide.

    Mammalian

    10 μg, 50 μg, 100 μg
  • IL-3 Protein, Human, Recombinant (His) [orb1959458]

    98.60%

    16.5 kDa (predicted)

    500 μg, 100 μg, 200 μg
  • IL-3 Protein, Human, Recombinant (His & Avi), Biotinylated [orb1960844]

    98.00%

    16.9 kDa (predicted) same as Tris-Bis PAGE result.

    500 μg, 100 μg, 1 mg
  • IL-3 Protein, Human, Recombinant (His & Avi) [orb1960845]

    98.00%

    16.9 kDa (predicted) same as Tris-Bis PAGE result.

    500 μg, 100 μg, 1 mg