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Catalog Number | orb1087548 |
---|---|
Category | Proteins |
Description | Human IL-3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1). |
Target | IL-3 |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_000579.2 |
UniProt ID | P08700 |
MW | 17.0 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 0.3 EU per μg |
Biological Origin | Human |
Biological Activity | Loaded Human IL-3 R alpha, Fc Tag (Cat. No. ILA-H5252) on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. ) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). |
Expression Region | Ala 20 - Phe 152 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_000579.2 |
Loaded Human IL-3 R alpha, Fc Tag on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. orb1087548) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 80-90 kDa after removal of the signal peptide. | |
Mammalian |
98.60% | |
16.5 kDa (predicted) |
98.00% | |
16.9 kDa (predicted) same as Tris-Bis PAGE result. |
98.00% | |
16.9 kDa (predicted) same as Tris-Bis PAGE result. |