Recombinant Human IL-3 Protein

SKU: orb3002361

Description

Recombinant Human Interleukin-3 is produced by our E.coli expression system and the target gene encoding Ala20-Phe152 is expressed with a 6His tag at the N-terminus.

Research Area

Immunology & Inflammation

Key Properties

• Expression System: E.coli
• Biological Activity: BLI: Loaded Human IL-3RA-Fc(Cat#orb2994092) on Protein A Biosensor, can bind Human IL-3(Cat#orb3002361) with an affinity constant of 3.89uM as determined in BLI assay. Loaded Human IL-3RA-Fc-Avi(Cat#orb2993952) on Protein A Biosensor, can bind Human IL-3(Cat#orb3002361) with an affinity constant of 3.74 uM as determined in BLI assay.
• Tag: N-6His
• Expression Region: Ala20-Phe152
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• MW: Predicted: 16.6 KDa. Observed: 16 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB,5%Sucrose,0.05%Tween80,4%Mannitol,pH 6.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-3; IL-3; Hematopoietic Growth Factor; Mast Cell Growth Factor; MCGF; Multipotential Colony-Stimulating Factor; P-Cell-Stimulating Factor; IL3

Quick Database Links

UniProt

P08700