You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb570286 |
---|---|
Category | Proteins |
Description | Human IL-23 R, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Gly 355 (Accession # Q5VWK5-1). |
Target | IL-23R |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_653302.2 |
UniProt ID | Q5VWK5 |
MW | 39.9 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.9 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,&Tag Free (Cat. No. ILB-H82W6) captured on Biotin CAP - Series S sensor Chip can bind Human IL-23 R, His Tag (SPR verified) (Cat. No. ) affinity constant of 3.39 nM as determined in a SPR assay (Biacore 8K) (QC tested). |
Expression Region | Gly 24 - Gly 355 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_653302.2 |
Biotinylated Human IL-23A & IL-12B Heterodimer Protein, His, Avitag & Tag Free captured on Biotin CAP-Series S sensor Chip can bind Human IL-23 R, His Tag (SPR verified) (Cat. No. orb570286) affinity constant of 3.39 nM as determined in a SPR assay (Biacore 8K) (QC tested).
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 37.4 kDa after removal of the signal peptide. | |
Mammalian |
98.00% | |
39.15 kDa (predicted). Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result. |
SDS-PAGE: 98.4%; SEC-HPLC: 99.6% | |
39.3 kDa (predicted) |
> 95% by SDS-PAGE. | |
Recombinant Human IL-23R Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Gly24-Asp353) of human IL-23R (Accession #Q5VWK5) fused with a 6��His Tag at the C-terminus. |