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Catalog Number | orb545751 |
---|---|
Category | Proteins |
Description | Human IL-21, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 162 (Accession # Q9HBE4-1). |
Target | IL-21 |
Conjugation | Unconjugated |
Tag | C-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH 7.5 |
Purity | 95% |
Protein Sequence | NP_068575.1 |
UniProt ID | Q9HBE4 |
MW | 41.9 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.9 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-21 R, His Tag at 2 µg/mL (100 µL/well) can bind Human IL-21, Fc Tag with a linear range of 0.2-8 ng/mL (QC tested). |
Expression Region | Gln 30 - Ser 162 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Research Area | Immunology & Inflammation |
Note | For research use only |
NCBI | NP_068575.1 |
Expiration Date | 6 months from date of receipt. |
Immobilized Human IL-21 R, His Tag (Cat. No. orb257627) at 2 μg/mL (100 μL/well) can bind Human IL-21, Fc Tag (Cat. No. orb545751) with a linear range of 0.2-8 ng/mL (QC tested).
Unconjugated | |
90% | |
41.9 kDa |