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Catalog Number | orb613659 |
---|---|
Category | Proteins |
Description | Human IL-2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 17.3 kDa |
Target | IL-2 |
UniProt ID | P60568 |
Protein Sequence | NP_000577.2 |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,&Fc, (Cat. No. ILG-H82F4) on SA Biosensor, can bind Human IL-2, His Tag (Cat. No. ) with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Ala 21 - Thr 153 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000577.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.3 kDa. The protein migrates as 18 kDa and 19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Biotinylated Human IL-2RB & IL-2RA & IL-2RG, Fc, Avitag & Fc, Avitag on SA Biosensor, can bind Human IL-2, His Tag (Cat. No. orb613659) with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).