Human IL-2 Protein, His Tag

• Catalog Number: orb613659
• Category: Proteins
• Description: Human IL-2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
• Target: IL-2
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• Protein Sequence: NP_000577.2
• UniProt ID: P60568
• MW: 17.3 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.3 kDa. The protein migrates as 18 kDa and 19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Loaded Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,&Fc, (Cat. No. ILG-H82F4) on SA Biosensor, can bind Human IL-2, His Tag (Cat. No. ) with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: Ala 21 - Thr 153
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_000577.2

Loaded Biotinylated Human IL-2RB & IL-2RA & IL-2RG, Fc, Avitag & Fc, Avitag on SA Biosensor, can bind Human IL-2, His Tag (Cat. No. orb613659) with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).