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Human IL-18 R1 Protein, Fc Tag
Catalog Number: orb257584
| Catalog Number | orb257584 |
|---|---|
| Category | Proteins |
| Description | Human IL-18 R1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # AAH69575). |
| Target | IL-18 R1 |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Purity | 95% |
| Protein Sequence | AAH69575 |
| UniProt ID | Q13478 |
| MW | 62.4 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 62.4 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Human IL-18 R1, Fc Tag (Cat. No. ) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 0.409 μM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
| Expression Region | Ala 19 - Arg 329 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | AAH69575 |
SDS-PAGE analysis of Human IL-18 R1 protein
Human IL-18 R1, Fc Tag (Cat. No. orb257584) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 0.409 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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