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Catalog Number | orb668885 |
---|---|
Category | Proteins |
Description | Human IL-17F, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1). |
Reactivity | Human |
Tag | N-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 16.8 kDa |
Target | IL-17F |
UniProt ID | Q96PD4 |
Source | Human IL-17F, His Tag (orb668885) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL-17F, His Tag with an affinity constant of 219 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Arg 31 - Gln 163 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_443104.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL-17F,Interleukin-17F,Cytokine ML-1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL-17F, His Tag (Cat. No. orb668885) with an affinity constant of 219 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Unconjugated | |
95% | |
16.8 kDa | |
Human IL-17F (H161R), His Tag (orb334906) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1 (H161R)). |
Unconjugated | |
95% | |
16.8 kDa | |
Cynomolgus IL-17F, His Tag (orb651991) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # G7P4V0-1). |
Unconjugated | |
90% | |
16.8 kDa | |
Mouse IL-17F, His Tag (orb653661) is expressed from human 293 cells (HEK293). It contains AA Arg 29 - Ala 161 (Accession # Q7TNI7-1). |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 15.7 kDa after removal of the signal peptide. The apparent molecular mass of His-IL17F is approximately 15-25 kDa due to glycosylation. | |
Mammalian |
98.00% | |
73.3 kDa (predicted) |