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Catalog Number | orb864189 |
---|---|
Category | Proteins |
Description | Human IL-15, His Tag is expressed from human 293 cells (HEK293). It contains AA Asn 49 - Ser 162 (Accession # P40933-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 14.8 kDa |
Target | IL-15 |
UniProt ID | P40933 |
Protein Sequence | Asn 49 - Ser 162 |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-15, His Tag (Cat. No. ) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc, (Cat. No. ILA-H82F4) with a linear range of 2-31 ng/mL (QC tested). |
Expression Region | Asn 49 - Ser 162 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.8 kDa. The protein migrates as 17-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human IL-15, His Tag (Cat. No. orb864189) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc, Avitag with a linear range of 2-31 ng/mL (QC tested).
98.00% | |
21.3 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
98.00% | |
21.3 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
98.00% | |
14.9 kDa (predicted); 15 kDa (reducing conditions) |
> 95% by SDS-PAGE. | |
Recombinant human IL-15 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Asn49-Ser162) of human IL-15 (Accession #NP_000576.1) fused with a Fc, 6��His Tag at the C-terminus. |