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Human IL-12B Protein, Fc Tag
Catalog Number: orb257708
| Catalog Number | orb257708 |
|---|---|
| Category | Proteins |
| Description | Human IL-12B, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499). |
| Target | IL-12B |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Purity | 95% |
| Protein Sequence | AAH67499 |
| UniProt ID | P29460 |
| MW | 61.3 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.3 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Immobilized Human IL-12 R beta 1, His Tag at 2 µg/mL (100 µL/well) can bind Human IL-12B, Fc Tag with a linear range of 0.039-0.625 µg/mL (QC tested). |
| Expression Region | Ile 23 - Ser 328 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| NCBI | AAH67499 |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-12B protein
Immobilized Human IL-12 R beta 1, His Tag (Cat. No. orb570283) at 2 μg/mL (100 μL/well) can bind Human IL-12B, Fc Tag (Cat. No. orb257708) with a linear range of 0.039-0.625 μg/mL (QC tested).
- Item 1 of 1
Human IL12A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743293]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide.
Mammalian
50 μg, 10 μg, 100 μg - Item 1 of 1
Human IL23A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743335]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation.
E.coli
10 μg, 50 μg, 100 μg 
Human IL12B Protein, Fc Tag [orb2697432]
>97%, determined by SDS-PAGE
This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells.
50 μg, 20 μgHuman IL12B Protein, Fc Tag [orb2697435]
>95%, determined by SEC-HPLC
This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells.
100 μg, 20 μgHuman IL12B Protein, Fc Tag [orb2697444]
>95%, determined by SDS-PAGE
This protein contains the p40 subunit of human IL12, termed asIL12B (NP_0021782) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus and expressed from HEK293 Cells.
1 mg, 100 μg
