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| Catalog Number | orb257707 |
|---|---|
| Category | Proteins |
| Description | Human IL-12A, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Arg 23 - Ser 219 (Accession # P29459). |
| Target | IL-12A |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Purity | 90% |
| UniProt ID | P29459 |
| MW | 49.2 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.2 kDa. The protein migrates as 57-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Expression Region | Arg 23 - Ser 219 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

SDS-PAGE analysis of Human IL-12A protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide. | |
Mammalian |
> 95% by SDS-PAGE. | |
Recombinant Human IL12A/NKSF1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg23-Ser219) of human IL12A/NKSF1 (Accession #NP_002178.2) fused with an Fc, 6��His Tag at the C-terminus. |
>95%, determined by SDS-PAGE | |
This protein contains the p35 subunit of human IL12, termed as IL12A (P29459) (Met 1-Ser 219) was fused with the Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells. |
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