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Human IL-10 Protein, His Tag
Description
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Target | IL-10 |
| Reactivity | Human |
| Tag | N-6×His |
| Molecular Weight | 19.5 kDa |
| Expression Region | Ser 19 - Asn 178 |
| Purity | 95% |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Disclaimer | For research use only |
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−Human IL10 Protein, His Tag [orb1471796]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 19.5 kDa after removal of the signal peptide. The apparent molecular mass of His-IL10 is approximately 15-25 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μg

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SDS-PAGE analysis of Human IL-10 protein

Immobilized Human IL-10, His Tag (Cat. No. orb334903) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc, Avitag with a linear range of 0.2-10 ng/mL (QC tested).
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Human IL-10 Protein, His Tag (orb334903)
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