Human IL-10 Protein, His Tag

• Catalog Number: orb334903
• Category: Proteins
• Description: Human IL-10, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1).
• Target: IL-10
• Conjugation: Unconjugated
• Tag: N-6×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• Protein Sequence: NP_000563.1
• UniProt ID: P22301
• MW: 19.5 kDa
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Immobilized Human IL-10, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc, (Cat. No. ILR-H82F6) with a linear range of 0.2-10 ng/mL (QC tested).
• Expression Region: Ser 19 - Asn 178
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_000563.1

SDS-PAGE analysis of Human IL-10 protein

Immobilized Human IL-10, His Tag (Cat. No. orb334903) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc, Avitag with a linear range of 0.2-10 ng/mL (QC tested).