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Human IL-10 Protein, His Tag
Catalog Number: orb334903
| Catalog Number | orb334903 |
|---|---|
| Category | Proteins |
| Description | Human IL-10, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1). |
| Reactivity | Human |
| Tag | N-6×His |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 19.5 kDa |
| Target | IL-10 |
| UniProt ID | P22301 |
| Source | Human IL-10, His Tag (orb334903) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Immobilized Human IL-10, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc, with a linear range of 0.2-10 ng/mL (QC tested). |
| Expression Region | Ser 19 - Asn 178 |
| Endotoxins | 1.0 EU per μg |
| NCBI | NP_000563.1 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC12 Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE). |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-10 protein
Immobilized Human IL-10, His Tag (Cat. No. orb334903) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc, Avitag with a linear range of 0.2-10 ng/mL (QC tested).
- Item 1 of 1
Mouse IL-10 Protein, His Tag [orb334904]
Unconjugated
92%
19.6 kDa
Mouse IL-10, His Tag (orb334904) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Ser 178 (Accession # NP_034678).
50 μg, 20 μg - Item 1 of 1
Cynomolgus IL-10 Protein, His Tag [orb1496244]
Unconjugated
90%
20.6 kDa
Cynomolgus IL-10 Protein, His Tag (orb1496244) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # P79338).
50 μg, 20 μg - Item 1 of 1
Cynomolgus IL-10 R alpha Protein, Fc Tag [orb1496248]
Unconjugated
90%
50.8 kDa
Cynomolgus IL-10 R alpha Protein, Fc Tag (orb1496248) is expressed from human 293 cells (HEK293). It contains AA His 22 - Asn 235 (Accession # XP_005579838.2).
1 mg, 100 μg - Item 1 of 1
Human IL-10 R alpha Protein, Fc Tag [orb1496310]
Unconjugated
95%
50.8 kDa
Human IL-10 R alpha Protein, Fc Tag (orb1496310) is expressed from human 293 cells (HEK293). It contains AA His 22 - Asn 235 (Accession # Q13651).
100 μg, 1 mg - Item 1 of 1
Human IL10 Protein, His Tag [orb1471796]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 19.5 kDa after removal of the signal peptide. The apparent molecular mass of His-IL10 is approximately 15-25 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg
