You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb752327 |
---|---|
Category | Proteins |
Description | Human IL-1 beta, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 117 - Ser 269 (Accession # P01584-1). |
Target | IL-1 beta |
Conjugation | Unconjugated |
Tag | N-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_000567.1 |
UniProt ID | P01584 |
MW | 43.8 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 43.8 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-1 RII, His Tag (Cat. No. IL2-H4226) at 5 μg/mL (100 μL/well) can bind Human IL-1 beta, Fc Tag (Cat. No. ) with a linear range of 4.9-39 ng/mL (QC tested). |
Expression Region | Ala 117 - Ser 269 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_000567.1 |
Immobilized Human IL-1 RII, His Tag (Cat. No. orb257591) at 5 μg/mL (100 μL/well) can bind Human IL-1 beta, Fc Tag (Cat. No. orb752327) with a linear range of 4.9-39 ng/mL (QC tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.The apparent molecular mass of IL1B-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |