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Description
Research Area
Protein Biochemistry
Images & Validation
−| Tested Applications | DOT |
|---|---|
| Application Notes |
Key Properties
−| Isotype | IgG |
|---|---|
| Conjugation | Cy5 |
| Purity | Human IgG whole molecule CY5 conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Human IgG whole molecule CY5 conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG and anti-Human Serum. |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Human IgG CY5 conjugated, Cy5 isotype control for flow cytometry

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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
DOT
Dot Blot
