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Description
Research Area
Protein Biochemistry
Images & Validation
−
| Tested Applications | FC, IHC, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human |
|---|---|
| Biological Origin | Human |
| Isotype | IgG |
| Conjugation | RPE |
| Purity | Human IgG whole molecule Phycoerythrin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Human IgG whole molecule Phycoerythrin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Human IgG and anti-Human Serum. |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01M Magnesium Chloride and 0.01M Beta-Mercaptoethanol |
| Concentration | 0.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Human IgG Phycoerythrin conjugated, Human IgG PE conjugated, isotype control for flow cytometry
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry







