Human IgG IgA IgM (H&L) Antibody Peroxidase Conjugated

SKU: orb347193

Description

Human IgG IgA IgM (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, IP: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat.
• Purity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgA and Human IgM. This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat Anti-Human IgG IgA IgM Antibody peroxidase Conjugated, Goat Anti-Human IgG IgA IgM Antibody peroxidase Conjugated, Goat Anti-Human IgGAM Antibody HRP Conjugated

Western Blot of Goat anti-Human IgG, IgA, IgM Peroxidase Conjugated Antibody. Lane 1: Human IgG. Lane 2: Human IgA. Lane 3: Human IgM. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: Appropriate banding pattern for Human IgG, IgA, and IgM is shown. Other band(s): Splice variants and isoforms.