Human IgG Fc Antibody Peroxidase Conjugated Pre-Adsorbed

• Catalog Number: orb347191
• Category: Antibodies
• Description: Human IgG F(c) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab)or Mouse serum proteins.
• Immunogen: Human IgG F(c) fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application notes: Anti-Human IgG F(c) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat anti-Human IgG F(c) peroxidase Conjugated Ant
• Note: For research use only

ELISA Results of purified Goat Anti-Human IgG F(c) Antibody Peroxidase Conjugated tested against purified Human IgG F(c) HRP. Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n orb346220) [Blue Line] and Human IgG (p/n orb346219) [Purple Line]. The working dilution is 1:33000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n orb348651).