Human IgG Fc Antibody

SKU: orb346649

Description

Human IgG F(c) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1-:1000-1:10000, WB: 1:1000 - 1:2000
• Reactivity: Human
• Application Notes:
  Human IgG F(c) antibody has been tested by ELISA, SDS-PAGE, and Western Blot. This product is suitable for immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3D8.H7.D4
• Immunogen: Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice.
• Purity: Anti-Human IgG F(c) Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG, Human Serum and Human F(c). No reaction was observed against Human F(ab).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Human IgG F(c) Antibody, Human IgG Fc Antibody, Ms-a-Human IgG Fc, Human IgG F(c) Antibody in Mouse, Human Secondary Antibody

Western Blot of Mouse anti-Human F(c) antibody. Lane 1: Human IgG (p/n orb346219). Lane 2: Human Fc (p/n orb346220). Lane 3: Human Fab (p/n orb346222). Lane 4: Human Fab2 (p/n orb346221). Lane 5: Human IgM (p/n orb2652752). Lane 6: Human IgM Fc5µ (p/n orb346226). Load: 50 ng per lane. Primary antibody: Anti-Human F(c) monoclonal antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted MW: ~28kDa. Observed MW: ~33kDa for Human Fc.