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Catalog Number | orb346649 |
---|---|
Category | Antibodies |
Description | Human IgG F(c) antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3D8.H7.D4 |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice. |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:1000 - 1:10000, WB: 1:1000 - 1:2000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Human IgG F(c) Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG, Human Serum and Human F(c). No reaction was observed against Human F(ab). |
Conjugation | Unconjugated |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Human IgG F(c) Antibody, Human IgG Fc Antibody, Ms Read more... |
Note | For research use only |
Application notes | Human IgG F(c) antibody has been tested by ELISA, SDS-PAGE, and Western Blot. This product is suitable for immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
DOT | |
Texas Red | |
Human IgG F(c) Texas Red was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
DOT, ELISA, SDS-PAGE, WB | |
Unconjugated | |
HUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
DOT | |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
DOT, ELISA, IHC, WB | |
Biotin | |
Human IgG F(c) fragment Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain. | |
Human |
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