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Human IgG Fc Antibody

SKU: orb346220

Description

Human IgG Fc Antibody

Research Area

Protein Biochemistry

Images & Validation

Tested ApplicationsDOT, ELISA, SDS-PAGE, WB
Application Notes
HUMAN IgG F(c) Fragment has been tested by SDS-Page and dot blot and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Key Properties

SourceHuman
Biological OriginHuman
IsotypeIgG F(c)
ConjugationUnconjugated
PurityHUMAN IgG F(c) was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(c). No reaction was observed against anti-Human IgG F(ab’)2 or anti-Papain.

Storage & Handling

StorageStore vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.029 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Human Immunoglobulin G F(c) Fragment, IgG Fc

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
DOT
Dot Blot
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

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1 mg
$ 510.00
DispatchUsually dispatched within 5-10 working days
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