Human IGFBP-4 Protein, Fc Tag (MALS verified)

• Catalog Number: orb1818947
• Category: Proteins
• Description: Insulin-like growth factor-binding protein 4 (IGFBP-4) is also known as IGF-binding protein 4, which contains an IGFBP domain and a thyroglobulin type-I domain. IGFBP-4 binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. IGFBP-4 is secreted by all colon cancer cells.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 52.4 kDa
• Target: IGFBP-4
• Source: Human IGFBP-4 Protein, Fc Tag (orb1818947) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IGF-I, His Tag, premium grade at 5 μg/mL (100 μL/well) can bind Human IGFBP-4 Protein, Fc Tag with a linear range of 1-20 ng/mL (QC tested).
• Expression Region: Asp 22 - Glu 258
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.4 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IGF-I, His Tag, premium grade (Cat. No. orb1146986) at 5 μg/mL (100 μL/well) can bind Human IGFBP-4 Protein, Fc Tag (Cat. No. orb1818947) with a linear range of 1-20 ng/mL (QC tested).