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| Catalog Number | orb1146966 |
|---|---|
| Category | Proteins |
| Description | Human IGFBP-3, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # P17936-1). |
| Target | IGFBP-3 |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| UniProt ID | P17936 |
| MW | 30.6 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.6 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Expression Region | Gly 28 - Lys 291 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Disease Biomarkers |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

Immobilized Biotinylated Human IGF-II, Avitag, Fc Tag at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (1 μg/well) plate can bind Human IGFBP-3, His Tag (Cat. No. orb1146966) with a linear range of 0.01-0.313 μg/mL (QC tested).
>90% as determined by SDS-PAGE | |
30.7 kDa |
> 85 % by SDS-PAGE. | |
Recombinant Human IGFBP-3 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Gly28-Lys291) of human IGFBP-3 (Accession #NP_000589.2) fused with a 6��His Tag at the N-terminus. |
>90% as determined by SDS-PAGE. | |
31.04 kDa |
>90% as determined by SDS-PAGE. | |
18.61 kDa |
Greater than 90.0% as determined by analysis by SDS-PAGE. | |
Escherichia Coli |
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