Human IGFBP-1 Protein, His Tag (MALS verified)

• Catalog Number: orb1786132
• Category: Proteins
• Description: IGFBP1 stands for insulin-like growth factor binding protein 1. It is a protein that binds to insulin-like growth factors (IGFs) and regulates their availability and activity within the body. IGFBP1 is part of a family of six IGFBPs (IGFBP1-6) that interact with IGFs to modulate their biological effects. IGFBP1 binds to IGFs (mostly IGF-1) with high affinity and prolongs their half-life in the circulation by protecting them from degradation. IGFBP1 has also been found to have IGF-independent functions, suggesting that it may have additional roles beyond regulating IGF signaling.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 27.2 kDa
• Target: IGFBP-1
• UniProt ID: P08833
• Source: Human IGFBP-1 Protein, His Tag (orb1786132) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Asn 259 (Accession # P08833).
• Expression System: HEK293
• Biological Activity: Immobilized Human IGF-I, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IGFBP-1 Protein, His Tag with a linear range of 0.01-0.156 μg/mL (QC tested).
• Expression Region: Ala 26 - Asn 259
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.2 kDa. The protein migrates as 32-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IGF-I, Fc Tag (Cat. No. orb257564) at 5 μg/mL (100 μL/well) can bind Human IGFBP-1 Protein, His Tag (Cat. No. orb1786132) with a linear range of 0.01-0.156 μg/mL (QC tested).