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Human Hyaluronoglucosaminidase 1 (HYAL1) ELISA Kit

Catalog Number: orb781204

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb781204
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronoglucosaminidase 1(HYAL1).
Tested applicationsELISA
ReactivityHuman
Range0.32-20 ng/mL
Assay Time3.5h
Concentration20 ng/mL
UniProt IDQ12794
Sensitivity0.121 ng/mL
Alternative namesLUCA1 ELISA Kit, NAT6 ELISA Kit, Hyaluronidase-1 E
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NoteFor research use only
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HYAL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HYAL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HYAL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HYAL1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Expiration DatePlease enquire.
Human Hyaluronoglucosaminidase 1 (HYAL1) ELISA Kit

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