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| Catalog Number | orb1736604 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Holo TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Holo TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Holo TC in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Concentration | 10 ng/mL |
| Application notes | standard: 10 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Holo TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Holo TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Holo TC in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.045 ng/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | Holo-TC |
| Research Area | Metabolism Research |
| Note | For research use only |
| Expiration Date | Please enquire. |
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Lingmiao Wang 1, Ailin Guo 2, Shuang Liang 3, Lingling Yu 3, Bai Shen 4, Zhihang Huang The association of serum hsa-miR-21-5p expression with the severity and prognosis of heart failure with reduced ejection fraction BMC Cardiovasc Disord, (2025)
Applications
ELISA
Reactivity
Human
