Human HLA-DRA(HLA class II histocompatibility antigen, DR alpha chain) ELISA Kit

• Catalog Number: orb2999137
• Category: Assays and Kits
• Description: Human HLA-DRA(HLA class II histocompatibility antigen, DR alpha chain) ELISA Kit
• UniProt ID: P01903
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-DRA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-DRA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-DRA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-DRA in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.053 ng/mL
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Alternative names: MHC class II antigen DRA;HLA-DRA;HLA-DRA1
• Note: For research use only