Human HLA Class II Histocompatibility Antigen Gamma Chain (CD74) ELISA Kit

SKU: orb866925

Description

Sandwich Human CD74 ELISA Kit

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD74. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD74. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD74, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD74 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.298 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD74 ELISA Kit, HLA class II histocompatibility antigen gamma chain ELISA Kit, CLIP ELISA Kit, DHLAG ELISA Kit, HLADG ELISA Kit, Ii ELISA Kit, II ELISA Kit, MHC HLA-DR gamma chain ELISA Kit, p33 ELISA Kit, Ia-gamma ELISA Kit, INVG34 ELISA Kit, CD74 antigen ELISA Kit, HLA-DR antigens-associated invariant chain ELISA Kit, HLA-DR-gamma ELISA Kit, Ia antigen-associated invariant chain ELISA Kit, Ia-associated invariant chain ELISA Kit