Human Histone Deacetylase 9 (HDAC9) ELISA Kit

SKU: orb777981

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 9(HDAC9).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HDAC9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HDAC9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HDAC9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HDAC9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.135 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HD7 ELISA Kit, HDAC ELISA Kit, HDAC7 ELISA Kit, HDAC7B ELISA Kit, HDAC9B ELISA Kit, HDAC9FL ELISA Kit, HDRP ELISA Kit, MITR ELISA Kit, Histone deacetylase-related protein ELISA Kit, MEF2-interacting transcription repressor MITR ELISA Kit, Histone deacetylase 7B ELISA Kit

Quick Database Links

UniProt

Q9UKV0