Human Histone Cluster 1, H4a (HIST1H4A) ELISA Kit

SKU: orb781603

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Cluster 1, H4a(HIST1H4A).

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIST1H4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIST1H4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIST1H4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HIST1H4A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

H4FA ELISA Kit, HIST2H4B ELISA Kit, HIST2H4C ELISA Kit, HIST2H4D ELISA Kit, HIST2H4E ELISA Kit, HIST2H4F ELISA Kit, HIST2H4G ELISA Kit, HIST2H4H ELISA Kit, HIST2H4I ELISA Kit, HIST2H4J ELISA Kit, HIST2H4K ELISA Kit, HIST2H4B ELISA Kit, HIST4H4 ELISA Kit, HIST2H4A ELISA Kit

Quick Database Links

UniProt

P62805