Human High Sensitivity Hepcidin 25 (hs-Hepc25) ELISA Kit

SKU: orb866898

Description

Sandwich Human hs-Hepc25 ELISA Kit

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-Hepc25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-Hepc25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-Hepc25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-Hepc25 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.63 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Hamp ELISA Kit, HEPC ELISA Kit, HEPC_HUMAN ELISA Kit, Hepc20 ELISA Kit, Hepc25 ELISA Kit, HEPCIDIN ELISA Kit, Hepcidin 20 ELISA Kit, Hepcidin 25 ELISA Kit, Hepcidin antimicrobial peptide ELISA Kit, Hepcidin-20 ELISA Kit, Hepcidin25 ELISA Kit, HFE2 ELISA Kit, HFE2B ELISA Kit, LEAP 1 ELISA Kit, LEAP-1 ELISA Kit, LEAP1 ELISA Kit, Liver expressed antimicrobial peptide ELISA Kit, Liver-expressed antimicrobial peptide 1 ELISA Kit, PLTR ELISA Kit, Putative liver tumor regressor ELISA Kit, ELISA Kit