Human High Affinity Nerve Growth Factor Receptor (TrkA/NTRK1) ELISA Kit

SKU: orb1146845

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High affinity nerve growth factor receptor(TrkA/NTRK1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to High affinity nerve growth factor receptor(TrkA/NTRK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High affinity nerve growth factor receptor(TrkA/NTRK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High affinity nerve growth factor receptor(TrkA/NTRK1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TrkA/NTRK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TrkA/NTRK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TrkA/NTRK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TrkA/NTRK1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 32 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NTRK1, MTC, TRK, TRKA, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A,

Quick Database Links

UniProt

P04629