You have no items in your shopping cart.
Cart summary
Item 1 of 1
Human HGF Protein, His Tag
Catalog Number: orb700640
| Catalog Number | orb700640 |
|---|---|
| Category | Proteins |
| Description | Human HGF, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1). |
| Target | HGF |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | Gln 32 - Ser 728 |
| UniProt ID | P14210 |
| MW | 81.6 kDa (53.7 kDa|27.9 kDa) |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.1 EU per μg |
| Biological Origin | Human |
| Biological Activity | Human HGF R, Fc Tag (Cat. No. MET-H5256) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade (Cat. No. ) with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
| Expression Region | Gln 32 - Ser 728 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
Human HGF R, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade (Cat. No. orb700640) with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Human IL6 Protein [orb752951]
Greater than 95.0% as determined by SDS-PAGE.
Sf9, Baculovirus cells
0.1 mg, 2 μg, 10 μgRecombinant Human HGF R/c-MET Protein, His Tag [orb1551050]
> 95% by SDS-PAGE.
Recombinant Human HGF R/c-MET Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Glu25-Thr932) of human HGF R/c-MET (Accession #NP_000236.2) fused with a 6��His Tag at the C-terminus.
100 μg, 50 μgRecombinant Human HGF/Hepatocyte Growth Factor Protein, His Tag [orb1550840]
> 95% by SDS-PAGE.
Recombinant Human HGF/Hepatocyte Growth Factor Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Gln32-Ser728) of human HGF/Hepatocyte Growth Factor (Accession #P14210) fused with a 6��His Tag at the C-terminus.
50 μgHuman HGFAC Protein, His Tag [orb2701646]
> 95%, determined by SDS-PAGE
This protein contains the human HGF activator precursor (NP_0015191) (Met 1-Ser 655) was expressed with a fused polyhistidine-tag at the C-terminus and expressed from HEK293 Cells.
10 μg, 20 μg
