Human HGF Protein, His Tag

• Catalog Number: orb700640
• Category: Proteins
• Description: Human HGF, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).
• Target: HGF
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• UniProt ID: P14210
• MW: 81.6 kDa (53.7 kDa|27.9 kDa)
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.1 EU per μg
• Expression Region: Gln 32 - Ser 728
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human HGF R, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade (Cat. No. orb700640) with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).