Human Hepatocyte Nuclear Factor 1 Alpha (HNF1a) ELISA Kit

SKU: orb778974

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Alpha(HNF1a).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HNF1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HNF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HNF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HNF1a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.064 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TCF1 ELISA Kit, HNF1 ELISA Kit, LFB1 ELISA Kit, LF-B1 ELISA Kit, MODY3 ELISA Kit, Transcription Factor 1,Hepatic ELISA Kit, Hepatic Nuclear Factor ELISA Kit, Albumin Proximal Factor ELISA Kit, Liver-specific transcription factor LF-B1 ELISA Kit

Quick Database Links

UniProt

P20823